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In vitro and pilot in vivo evaluation of a bioactive coating for stent grafts based on chondroitin sulfate and epidermal growth factor

Lequoy, Pauline et Savoji, Houman et Saoudi, Bachir et Bertrand-Grenier, Antony et Wertheimer, Michael R. et De Crescenzo, Gregory et Soulez, Gilles et Lerouge, Sophie. 2016. « In vitro and pilot in vivo evaluation of a bioactive coating for stent grafts based on chondroitin sulfate and epidermal growth factor ». Journal of Vascular and Interventional Radiology, vol. 27, nº 5. 753-760.e753.

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Résumé

Purpose: To evaluate the potential of a bioactive coating based on chondroitin sulfate (CS) and tethered epidermal growth factor (EGF) for the improvement of healing around stent-grafts (SG). Methods: The impact of the bioactive coating on cell survival was tested in vitro on human vascular cells using polyethylene terephtalate films (PET) as a substrate. After being transferred onto a more “realistic” material (expanded poly(tetrafluoroethylene), ePTFE), the durability and mechanical behavior of the coating in addition to cell survival were studied. Preliminary in vivo testing was performed in a canine iliac aneurysm model reproducing type I endoleaks (3 animals with 1 control and 1 bioactive SG for each). Results: The CS and EGF coatings significantly increased survival of human smooth muscle cells and fibroblasts, compared with bare PET or ePTFE (P<.05). The coating also displayed good durability over 30 days according to ELISA and cell-survival tests. The coating did not affect the mechanical properties of ePTFE and was successfully transferred onto commercial SG for in vivo testing. According to CT-scan and macroscopic examinations, no difference was observed in endoleak persistence at three months, but the bioactive coating deposited on the abluminal surface of the SG (exposed to the vessel wall) increased the percentage of healed tissue in the aneurysm. Moreover, no adverse effect such as neointima formation or thrombosis was observed. Conclusion: The bioactive coating promoted in vitro cell survival, displayed good durability and was successfully transferred onto a commercial SG. Preliminary in vivo results suggest improved healing around bioactive SG.

Type de document: Article publié dans une revue, révisé par les pairs
Professeur:
Professeur
Lerouge, Sophie
Affiliation: Génie mécanique
Date de dépôt: 10 mai 2016 16:27
Dernière modification: 30 mai 2017 20:35
URI: http://espace2.etsmtl.ca/id/eprint/12584

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