La vitrine de diffusion des publications et contributions des chercheurs de l'ÉTS

Characterization and comparison of N-, O-, and N+O-functionalized polymer surfaces for efficient (HUVEC) endothelial cell colonization


Téléchargements par mois depuis la dernière année

Plus de statistiques...

Boespflug, Gaël et Maire, Marion et De Crescenzo, Gregory et Lerouge, Sophie et Wertheimer, Michael R.. 2017. « Characterization and comparison of N-, O-, and N+O-functionalized polymer surfaces for efficient (HUVEC) endothelial cell colonization ». Plasma Processes and Polymers, vol. 14, nº 7.
Compte des citations dans Scopus : 2.

Lerouge S 2017 14336 Characterization and comparison of N O and.pdf - Version acceptée
Licence d'utilisation : Tous les droits réservés aux détenteurs du droit d'auteur.

Télécharger (937kB) | Prévisualisation


Surface modifications are often required to enhance cell adhesion and growth around implanted biomaterials. This study compares various functionalization processes in their ability to create high densities of oxygen‐ and/or nitrogen‐containing functional groups, mostly on a polymeric biomaterial, polyethylene terephthalate (PET). Primary amine (NH2)‐rich surfaces were prepared by low‐pressure plasma‐polymerization (L‐PPE:N), plasma modification (functionalized PET, “PETf”), chemical vapour deposition (Parylene diX AM), and grafting of polyallylamine (PAAm). Plasma polymerization was also used to obtain oxygen‐rich (L‐PPE:O) as well as hybrid (L‐PPE:O,N) films, which were respectively compared to oxygen‐rich tissue culture polystyrene (TCP) and hybrid (Primaria™) culture plates. Compositions and bond types were studied by X‐ray photoelectron spectroscopy. Finally, the effect of each surface on cell adhesion and growth was assessed using human umbilical vein endothelial cells (HUVECs). Amine‐containing surfaces manifested a wide [NH2] range, up to 8.9%. Hybrid surfaces, Primaria™ and L‐PPE:O,N, showed lower [NH2] in spite of high [N], suggesting more varied and complex functionalities. Except for Parylene, all O‐ and NH2‐rich surfaces promoted HUVEC adhesion and growth similarly, despite differing chemical compositions. Primaria™ showed the best cell behavior, but L‐PPE:O,N did not reproduce this apparent synergistic effect. To conclude, both N‐ and O‐rich surfaces displayed good cell‐colonization properties, particularly plasma polymers, while “hybrid” surfaces appear somewhat ambiguous and call for further investigation.

Type de document: Article publié dans une revue, révisé par les pairs
Lerouge, Sophie
Affiliation: Génie mécanique
Date de dépôt: 23 janv. 2017 15:56
Dernière modification: 18 oct. 2018 17:00
URI: http://espace2.etsmtl.ca/id/eprint/14336

Actions (Authentification requise)

Dernière vérification avant le dépôt Dernière vérification avant le dépôt