Probiotic disruption of quorum sensing reduces virulence and increases cefoxitin sensitivity in methicillin-resistant Staphylococcus aureus

Cella, Monica Angela, Coulson, Thomas, MacEachern, Samantha, Badr, Sara, Ahmadi, Ali, Tabatabaei, Mahdis Sadat, Labbe, Alain et Griffiths, Mansel William. 2023. « Probiotic disruption of quorum sensing reduces virulence and increases cefoxitin sensitivity in methicillin-resistant Staphylococcus aureus ». Scientific Reports, vol. 13, nº 1.
Compte des citations dans Scopus : 8.

Prévisualisation

PDF Ahmadi-A-2023-26313.pdf - Version publiée Licence d'utilisation : Creative Commons CC BY. Télécharger (5MB) | Prévisualisation

Résumé

Therapies which target quorum sensing (QS) systems that regulate virulence in methicillin-resistant Staphylococcus aureus (MRSA) are a promising alternative to antibiotics. QS systems play a crucial in the regulation of MRSA antibiotic resistance, exotoxin production, antioxidant protection and immune cell evasion, and are therefore attractive therapeutic targets to reduce the virulence of a pathogen. In the present work the the effects of bioactive peptides isolated from two strains of lactic acid bacteria were tested against antibiotic resistance, carotenoid production, resistance to oxidative killing and biofilm structure in two clinical MRSA isolates. The results obtained from fractional-inhibitory concentration assays with bulk and semi-purified bioactive molecules showed a significant synergistic effect increasing cefoxitin mediated killing of MRSA. This was coupled to a six-fold decrease of the major membrane pigment staphyloxanthin, and a 99% increase in susceptibility to oxidative stress mediated killing. Real-time quantitative PCR analysis of the QS-genes agrA and luxS, showed differential expression between MRSA strains, and a significant downregulation of the hemolysin gene hla. Light microscopy and scanning electron microscopy revealed alteration in biofilm formation and clustering behavior. These results demonstrate that bioactive metabolites may be effectively applied in tandem with beta-lactam antibiotics to sensitize MRSA to cefoxitin. Moreover, these results shown that several key QS-controlled virulence mechanisms are diminished by probiotic metabolites.

Type de document:	Article publié dans une revue, révisé par les pairs
Professeur:	Professeur Ahmadi, Ali
Affiliation:	Génie mécanique
Date de dépôt:	06 avr. 2023 21:57
Dernière modification:	12 avr. 2023 19:07
URI:	https://espace2.etsmtl.ca/id/eprint/26313

Actions (Authentification requise)

Dernière vérification avant le dépôt