Turcitu, Tatiana, Champagne, Kyle, Le, Andy V. et Fenech, Marianne. 2025. « The role of diamide and glutaraldehyde in modulating red blood cell viscosity and deformability ». Communication lors de la conférence : CSME-CFDSC-CSR 2025 International Congress (Montreal, QC, Canada, May 25-28, 2025).
Prévisualisation |
PDF
251 - The role of diamide and glutaralde.pdf - Version publiée Licence d'utilisation : Tous les droits réservés aux détenteurs du droit d'auteur. Télécharger (101kB) | Prévisualisation |
Résumé
Red blood cell (RBC) deformability is vital for their functionality, enabling them to navigate narrow capillaries, ensure efficient oxygen delivery, and maintain proper blood flow dynamics. Reduced deformability can lead to blockages, impaired oxygen transport, and various vascular complications. Additionally, alterations in RBC deformability are linked to diseases such as sickle cell anemia, hereditary spherocytosis, diabetes, and cardiovascular disorders. In vitro studies on RBC deformability often involve modifying RBCs to simulate pathological rigidity, with diamide and glutaraldehyde being the primary agents employed for this purpose. Despite their widespread use, the dose-dependent effects of these agents on RBC mechanics remain incompletely understood.This study aims to elucidate the effects of different concentrations of diamide (10-200 µM) and different concentrations of glutaraldehyde (0.0001-2%) on RBC viscosity, deformability and morphology at 20% hematocrit (HCT). Viscosity is measured using a viscometer, while deformability is assessed through ektacytometry, a laser diffraction-based technique. Sphericity measurements complement the investigation to provide a comprehensive understanding of the impact of these agents. Results reveal that diamide treatment significantly affects RBC viscosity and sphericity, leading to a progressive increase across all tested concentrations as the concentration of diamide increases. These changes are accompanied by a biphasic response in deformability, as the maximum elongation index (EImax) decreases up to 100 µM before gradually increasing at higher concentrations (120-200 µM). This indicates that diamide induces both morphological and mechanical changes, resulting in heightened RBC rigidity and altered flow properties.In contrast, glutaraldehyde-treated RBCs display distinct behavior. Viscosity and sphericity remain consistent across all tested concentrations, indicating that glutaraldehyde's effects are predominantly mechanical rather than morphological. However, EImax decreases progressively with increasing glutaraldehyde concentrations, highlighting its impact on RBC deformability. This divergence in response between diamide and glutaraldehyde underscores the importance of understanding the specific mechanisms through which these agents influence RBC properties.The study addresses a significant knowledge gap in hemorheology by systematically exploring the dose-dependent effects of diamide and glutaraldehyde. These findings contribute to the broader understanding of RBC rigidity and its implications for blood flow and oxygen delivery. Additionally, the insights gained have potential applications in the development of therapeutic strategies for diseases associated with altered RBC deformability. By providing a detailed characterization of these agents, this research advances our knowledge of RBC mechanics and supports further exploration in the fields of hemorheology and microcirculation.
| Type de document: | Communication (Communication) |
|---|---|
| Informations complémentaires: | Progress in Canadian Mechanical Engineering, Volume 8. Co-chairs: Lucas A. Hof, Giuseppe Di Labbio, Antoine Tahan, Marlène Sanjosé, Sébastien Lalonde and Nicole R. Demarquette. |
| Date de dépôt: | 18 déc. 2025 14:56 |
| Dernière modification: | 18 déc. 2025 14:56 |
| URI: | https://espace2.etsmtl.ca/id/eprint/32282 |
Actions (Authentification requise)
 |
Dernière vérification avant le dépôt |