Hamouda, Inès, Da Rocha Moreau, Maïlys, Basso, Lisa, Trombotto, Stéphane, Montembault, Alexandra, David, Laurent et Lerouge, Sophie.
2026.
« Towards tunable mechanical properties of in situ gelling chitosan hydrogels: Impact of macromolecular structure including pattern of acetylation ».
Carbohydrate Polymers, vol. 373.
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Résumé
Injectable in situ gelling physically crosslinked chitosan (CH) hydrogels allowing cell encapsulation are appealing for biomedical applications. However, the variability of the CH source is one of the major difficulties in ensuring their reproducibility. We investigated here the effect of the CH degree and pattern of acetylation (DA and PA) on its final physicochemical properties. Hydrogels made from re-acetylated CH presenting statistical repartition of repeat units along the chains, with DA of 35, 10 and 1 %, were compared to a commercial CH with DA of 10 % differing by their PA. WAXS analysis showed different crystalline signatures depending on the PA of CH samples. Rheometry revealed faster gelation kinetics, but lower final modulus for hydrogels made of commercial versus statistical CH of same DA. Both also differed in terms of porosity and stability in solution. Hydrogel stiffness from 60 to 0.3 kPa were obtained by varying DA and PA. Hydrogels with the lowest DA were the most stable in solutions. Encapsulated L929 fibroblasts presented similar increasing metabolic activity over 7 days of culture within all hydrogels. This work demonstrates the relevance of controlling chitosan DA and PA for the generation of reproducible hydrogels with tunable final mechanical properties for targeted bio-applications.
| Type de document: | Article publié dans une revue, révisé par les pairs |
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| Professeur: | Professeur Lerouge, Sophie |
| Affiliation: | Génie mécanique |
| Date de dépôt: | 03 déc. 2025 18:47 |
| Dernière modification: | 10 janv. 2026 16:12 |
| URI: | https://espace2.etsmtl.ca/id/eprint/33091 |
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