Goodarzi, Hamid, Islam, Mohammad Mirazul, Zamani-Roudbaraki, Mostafa, Muhayimana, Solange, Lerouge, Sophie, Sjöström, Dick J., Islam, Rakibul, Saha, Amrita, Nilsson, Per H., Alarcon, Emilio I., Robert, Marie-Claude, Boutopoulos, Christos et Griffith, May.
2026.
« Supercritical carbon dioxide-peracetic acid treatment inactivates low-level bacterial inocula in collagen analogue-phosphorylcholine hydrogels while preserving functional properties ».
ACS Biomaterials Science & Engineering.
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Résumé
Terminal sterilization of hydrogels is challenging because their high-water-content, chemical structures, and cell-friendly properties are often altered by conventional sterilization methods. Hydrogel implants comprising collagen-like peptide (CLP), polyethylene glycol (PEG), and poly (2-methacryloyloxyethyl phosphorylcholine) (MPC) showed promise in promoting corneal regeneration in preclinical animal studies. However, before these CLP-PEG-MPC implants can be used clinically, they must be sterilized effectively to ensure patient safety. Here, we evaluated supercritical carbon dioxide (scCO2) with peracetic acid (PAA) as a potential sterilization technique for hydrogels. CLP-PEG-MPC was treated with scCO2 containing PAA and compared against 15 kGy of electron beam irradiation (E-beam) and chloroform (CF) sterilization. Several samples were spiked with 200 colony-forming units (CFU) of either Pseudomonas aeruginosa or Staphylococcus aureus prior to sterilization to compare the bactericidal efficacy of the three sterilization methods. Only scCO2-PAA effectively inactivated all the inoculated bioburden. Non-spiked hydrogels were also sterilized and then compared for complement activation, changes to their physical and mechanical properties, and biocompatibility. Optical clarity was unchanged regardless of sterilization mode. scCO2-PAA did not alter the mechanical properties of the hydrogels but resulted in a modest lowering of corneal epithelial cell proliferation, while E-beamed hydrogels showed reduced mechanical strength. In conclusion, newer scCO2-PAA terminal sterilization preserves the functional properties of soft hydrogels, such as CLP-PEG-MPC, including transparency, which is needed for corneal implants.
| Type de document: | Article publié dans une revue, révisé par les pairs |
|---|---|
| Chercheur(-euse): | Chercheur(-euse) Lerouge, Sophie |
| Affiliation: | Génie mécanique |
| Date de dépôt: | 04 sept. 2026 20:46 |
| Dernière modification: | 26 sept. 2026 20:51 |
| URI: | https://espace2.etsmtl.ca/id/eprint/34279 |
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